Psilocybin spore syringe are used for inoculating substrates to produce mushrooms. They can also be used to conduct microscopic research. They are generally preferred to spore prints (note: the use of spore syringes other than for microscopy is currently illegal in Canada).
Spore syringes are sealed with a cap, known as a Luer lock. To open it, hold the syringe with its Luer lock pointing up. Twist the cap counter-clockwise with your hand and remove it. The needle should still be sterile, so detach it from its sterile package (leaving the protective cap on). Attach it to the syringe and shake it for a few seconds.
The syringe is now ready for inoculation. This process is best conducted in a clean environment, ideally in a laminar flow hood, to reduce contamination from dust and debris. It is recommended to wear latex gloves and a mask, and make sure your workspace is clean to avoid infection. It is important to sterilize the syringe, water and shot glass before using it.
Inoculating a substrate with psilocybin spores is simple, but care must be taken not to overdose the substrate. For example, if you inoculate a jar of substrate with too many spores, the mycelium may grow too quickly and die before fruiting, producing only mold instead of the desired mushroom. To avoid this, only squirt around 4ml of spore solution over multiple holes punched into the jar’s lid. If this is done correctly, the spores will start to form a lace-like network of fungal threads called mycelium, and within a week should begin to fruit. Psilocybin spore syringe
